CSP® actively plays a role in membrane stabilization and protects against premature sperm capacitation. Equipped with a highly effective pH buffer system, protective antioxidants and sperm-compatible antibacterial agents, star series extenders preserve fertilizing capacities over a wide temperature range during long-term storage.Ĭell Shield Protection (CSP®) is a proprietary additive in Androstar® Plus and Androstar® Premium, which provides unparalleled protection for sperm when exposed to temperature fluctuations or physical stress. Minitube star series extenders ( Novistar®, Androstar® Plus and Androstar® Premium) are designed to enhance the flexibility of semen use. In conclusion, the most suitable concentration of Minitube Equex paste in the current protocol was 1.9% supplemented with 9.0% glycerol in TEY-based freezing extender III, based on the conformity between data from manual guides and the observed sperm motility characteristics results.The Minitube star series: superior long-term preservation Moreover, after thawing at 1 h, LIN (linearity) in Group IV was higher than in Group II (P < 0.05), but did not differ from the other groups. In Group IV after thawing at 0 h, rapid velocity and the velocity curved line were significantly higher than in Groups II and III (P < 0.05) but did not differ from Group I. After freezing and thawing, sperm motility characteristics were evaluated by Sperm Class Analyzer® incubated at 37 ☌ for 0 (10 min), 1 and 2 h post-thawing. Groups II, III and IV were the experiment groups, and contained different concentrations of Minitube Equex paste (Group II: 1.5% Group III: 1.7% and Group IV: 1.9%) added to the freezing extender III. Group I was the control and included only 1.5% Nova Equex STM paste. Each ejaculate was aliquoted and cryopreserved in base freezing extender III as Tris-citrate egg yolk (TEY) extender plus 9.0% glycerol classified into four groups. Fifteen ejaculates from 12 mature boars were collected by the glove-hand method. Paste (Minitube, Tiefenbach, Germany) for boar semen cryopreservation in comparison with Nova Equex STM paste (control). The aim of the present study was to determine the optimal concentration of Minitube Equex However, Equex paste produced by Nova Chemical Sales Inc. Įquex paste is a non-permeating cryoprotective agent (CPA) that improved post-thaw survival of spermatozoa during boar semen cryopreservation. Tummaruk, "Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics", Veterinarska stanica, vol.52, br. Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics. Suwimonteerabutr J, Nuntapaitoon M, Tummaruk P. 'Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics', Veterinarska stanica, 52(3), str. Suwimonteerabutr, J., Nuntapaitoon, M., i Tummaruk, P. "Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics." Veterinarska stanica 52, br. Suwimonteerabutr, Junpen, Morakot Nuntapaitoon i Padet Tummaruk. "Determination of the optimal concentration of Minitube Equex paste for boar semen cryopreservation based on sperm motility characteristics." Veterinarska stanica, vol.
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